Immunohistochemistry Detection Method of Rejection Reaction of Human Umbilical Cord Derived Mesenchymal Stem Cell on Rat Sciatic Nerve Tissue

Ria Margiana, Ahmad Aulia Jusuf, Silvia Werdhy Lestari


Abstract:Human umbilical cord derived mesenchymal stromal cells (UC-MSCs) that are accessible from cell banks can be actuated to separate into different cell sorts, accordingly making them handy potential hotspots for cell-based treatments. In injured peripheral nerves, Schwann cells (SCs) add to useful recuperation by supporting axonal recovery and myelin remaking. Here, we first exhibit a framework to induce UC-MSCs to separate into cells with SC properties (UC-SCs) by treatment with β-mercaptoethanol took after by retinoic corrosive and an arrangement of particular cytokines. The UC-SCs are morphologically like SCs and express SC markers, including P0, as evaluated by immunocytochemistry and converse interpretation polymerase chain response. Transplantation of UC-SCs into transected sciatic nerves in grown-up rats improved nerve recovery. The viability of UC-SCs for axonal recovery was tantamount to that of legitimate human SCs taking into account histological criteria and useful recuperation. Immunohistochemistry and immunoelectron microscopy additionally showed myelination of recovered axons by UC-SCs. These discoveries demonstrate that cells with SC properties and with the capacity to bolster axonal recovery and reproduce myelin can be effectively actuated from UC-MSCs to advance practical recuperation after peripheral nerve injury. This framework might be pertinent for the advancement of cell-based treatments.

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