Biological Activities of Red and Green Algae from Visakhapatnam Coast

Satyalakshmi Siragam


Objective: Determination of antibacterial, antioxidant, anti-inflammatory and anti-tumor activity of green and red algae collected from Visakhapatnam sea coast.

Methods: In vitro methods are used for determination of biological activities of algal extracts. Diffusion method of microbiological assay was used for estimation of antibacterial activity. Reducing power and free radical scavenging method for determination of antioxidant activity of algae. In vitro anti-inflammatory activity was determined by egg albumin and erythrocyte stabilization. HeLa (Cervical carcinoma) cell lines are used for determination of anti-tumor activity.

Results: Red algae Gracilaria corticata exhibited good antibacterial activity against selected test microorganisms. In vitro free radical scavenging method is used for determination of antioxidant activity of Caulerpa taxifolia and exhibited 48.76±0.08% of inhibition at 200 µg/mL. Gracilaria corticata exhibited good anti-inflammatory activity of 58% which was determined by in vitro method of erythrocyte stabilization and 66.75% by egg albumin method. Green algae Caulerpa taxifolia exhibited (IC50 = 32±1.2) remarkable antitumor activity on HeLa cell lines than control.

Conclusion: Among two organic solvents used for extraction of active principles from marine algae, methanolic extracts showed better biological activity than the chloroform extract. Green algae Caulerpa taxifolia exhibited significant antioxidant and anti tumor activity than remaining two algal species. Red algae Gracilaria corticata showing remarkable anti-inflammatory activity in both the in vivo methods. This study expands the medicinal importance of marine algae collected from coastal regions like Visakhapatnam.

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Zornitsa K, Julia S, Hristo N, Kamen S, Iva T, Stefka DK, Simeon P. Antibacterial, antiviral and cytotoxic activities of some red and brown seaweeds from the Black Sea. Botanica. Marin. 2008; 54:80-86.

De Fellcio R, Dealbuquerque S, Young MC, Yokoya NS, Debonsi HM. Trypanocidal, leishancidal and antifungal potential from marine red algae Bastrychiatenella. J of Pharm and Biomed Analysis. 2010; 52: 763-769.

Bouhlal R, Haslin C, Chermann JC, Colliec- jouault S, Riadi H, Bourgougnon N. Antiviral activity of sulphated polysaccharides isolated from Sphaerococcus coronopifolius (Rhodophytha Cigartinales) and Boergeseniellathyoides (Rhodophytha, Ceramiales). Mar Drugs. 2011; 9:1187-1209.

Bouhlal R, Riadi H, Bourgougnon N, Antiviral activity of extract of Rodophyceae from Morocco. African J of Biotechnol. 2010; 9:7968-7975.

Kim SK, Thomas NV, Li X. Anticancer compounds from marine macroalgae and their application as medicinal foods. Adv Food Nutr Res. 2011; 64: 213-224.

Rahul S, Shree T, Ashwani R, Tribhuban M. Cyan bacteria: an emerging source for drug discovery. The J of Antibio. 2011; 64:401-412.

Marijana K, Branislav R, Tatjana S. Biological activities of two macroalgae from Adriatic coast of Montenegro. Saudi Journal of Biological Sciences. 2015; 22:390-397.

Dayong S, Jing L, Shuju G, Lijun H. Antithrombotic effect of bromophenol, the alga-derived thrombin inhibitor. J Biotechnol. 2008; 136: 577-588.

Subba Rangaiaha G, Lakshmi P, Sruthikeerthia K. Antimicrobial activity of the crude extracts of Chlorophycean seaweeds Ulva, Caulerpa and Spongomorpha sps against clinical and phytopathogens. Drug Inven Today. 2010; 2: 311-314.

Jayaprakash GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed extracts on peroxidation models in vitro. J of Agri Food Chem. 2001; 55: 1018-1022.

Oyaizu M. Studies on products of browning reaction prepared from glucosamine. Jap J Nutr. 1986; 44: 307-314.

Zhang WW, Duan XJ, Huang HL, Zhang Y, Wang BG. Evaluation of 28 marine algae from the Quingdao coast for anti-oxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and sub fractions derived from Symphyocladia latiuscula (Rhodomelaceae). J Appl Phy. 2007; 19:97-108.

Vana JR, Botting RM, New insight into the mode of action of anti-inflammatory drugs. Inf Res. 1995; 44:1-10.

Bhattacharya S, Chandra S, Dey P, Chatterjee P. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed. 2012; 2: 178-180.

Kameyama Y, Yamashita K, Kobayashi K, Hosokawa M, Chiba K. Functional characterization of SLCO1B1 (OATP-C) variants, SLCO1B1*15 and SLCO1B1*15+C11007G by using transient expression systems of HeLa and HEK293 cells. Pharmaco and Geno. 2005; 15:513-522.

Vlachos V, Critchley AT, Holy A. Establishment of a protocol for testing antimicrobial activity in southern African macro algae. Microbios. 1996; 88:115-123.

Ozdemir G, Karabay NU, Dalay MC, Pazarbasi B. Antibacterial activity of volatile component and various extracts of Spirulinaplatensis. Phytother Res. 2004; 18: 754-757.

Karabay-Yavasoglu NU, Atakan S, Guven O, Zerrin H. Antimicrobial activity of volatile components and various extracts of the red alga Janiarubens. Phytother Res. 2007; 21: 153-156.

Pane G, Cacciola G, Giacco E, Mariottini GL, Coppo E. Assessment of the antimicrobial activity of algae extracts on bacteria responsible of external Otitis. Mar Drugs. 2015; 13: 6440- 6452.

Kandhasamy M, Arunachalam KD. Evaluation of in vitro antibacterial property of seaweeds of southeast coast of India. African J of Biotechnol. 2008; 7: 1958-1961.

Meenakshi S, Manicka GD, Tamil mozhi S, Arumugam M, Balasubramanian T. Total flavanoid and in vitro antioxidant, antibacteria from seaweeds of Rameshwaram coast. Global J Pharm. 2009; 3: 59-62.

Cox S, Abu-Ghannam N, Gupta S. An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds. Int Food Res J. 2010; 17: 205-220.

Srivastava N, Saurav K, Mohanasrinivasan V, Kannabiran K, Singh M. Antibacterial potential of macroalgae collected from the Madappam coast, India Bri J Pharm Toxi. 2010; 1:72-76.

Tariq M, Athar J, Ara V, Sultana S, Ehteshamul-Haque M. Ahmad Biochemical evaluation of antioxidant activity in extracts and polysaccharide fractions of seaweeds. Global J Environ Sci Manage. 2015; 1:47-62.

Ben FR, Frikha F, Kammoun M, Hammami N, Mchirgui RA, Belbahri L, Gargouri Y, Miled N. Chemical composition and some biological activities of marine algae collected in Tunisia. Ciencias Marinas. 2011; 37:113-124.

Paul JP. Anti-inflammatory activity of methanolic extract of Gracilaria corticata (red seaweed) in hare island, Thoothukudi, Tamilnadu, India. Int J of Pharm Deve & Technol. 2017; 7:21-24.

Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziura P, Nkeh-Chungag BN, Iputo JE. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J of Medici Plant Res. 2010; 4: 789-795.

Jatap VA, Agasimundim YS, Jayachandran E, Sathe BS. In vitro anti-inflammatory activity of 2-amino-3-(substituted benzylidinecarbohydrazide)-4,5,6,7 tetrahydrobenzothiophene. J of Pharm Res. 2011; 4: 378-379.

Rabia A, Mahboba N, Nazlin K. Anticancer and antioxidant activities of some algae from Western Libyan Coast. Pharmacol Toxi. 2016; 1: 9-18.

Seyed HM, Karkhane YM, Mashinchian MA, Ghasempour AR. In vitro investigating of anticancer activity of focuxanthin from marine brown seaweed species. Global J Environ Sci Manage. 2018; 4: 81-90.


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