Human sperm DNA integrity : A difference of storage temperature and period of freeze-drying process

silvia werdhy lestari, ahmad silalahi, hamdani lunardi, agustinus agustinus

Abstract

The cryopreserve technique using liquid nitrogen is used to preserve human sperm. Nevertheless, this technique has several disadvantages, such as bacterial/viral contamination and its expensive cost. Freeze-drying process offers more simple and cheaper of sperm storage technique. This study aimed to investigate the effect of storage temperature and period of freeze-dried human sperm on DNA integrity. Human sperm samples of 15 donors were prepared with simple washing techniques. Post-washing samples (T0) were divided into 4 groups with the same volume. The four group of samples carried on the freeze-drying process and stored based on different temperature and period: 4°C for 1 week (T1), room temperature (24-25ºC) for 1 week (T2), 4°C for 3 months (T3), and room temperature for 3 months (T4). Sperm DNA integrity was analyzed before and after the storage period of each group. There was a statistically significant difference of sperm DNA integrity of freezes - dried at 4°C in 1 week versus 3 months (T1 vs T3), at room temperature in 1 week versus 3 months (T2 vs T4) (p <0.05) and between both temperatures in 3 months (T3 vs T4) (p<0.05). In conclusion, temperature at 4°C and period in 1 week is the best temperature and storage to store freeze-dried human sperm, based on sperm DNA integrity.

Keywords: freeze-drying process, human sperm, sperm DNA integrity

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