Cloud Point Extraction for the Separation, Preconcentration and Spectrophotometric Determination of Trace Copper (II) In Herbal Plants Samples Using New Synthesized Reagent

Abstract

A new Cloud point extraction method was showed determination and preconcentration of copper in plant and herbs. The final products of analysis are complex of Cu (II) with new reagent is 6-(4-bromophenylazo) m-anisidine [6-(4BrPAA)]  in Triton X-114. Centrifuge is used after that, then the surfactant-rich phase is diluted by ethanol (1.5) mL after separation. Then measurement of the copper was done by spectrophotometry at 512 nm. The complexing agent concentration, pH, Triton X-114, centrifuge rate, time and temperature are important analytical parameters. The used analytical methods in the study are included limits of detection, molar absorptivity, linear range, quantification and sandell sensitivity, factors of improvement and preconcentration. Linear range was (0.007-1.50) μg mL^-1 of Cu (II). The quantification limits and detection were (0.0199 and 0.0059) μg mL^-1 of Cu (II), consequently. The cations and anions influence was examined; it is represent the method which used for evaluation the copper amount in the herbal plants samples.

Keywords: Copper (II); 6-(4-bromo phenylazo) m-anisidine [6-(4BrPAA)]; Triton X-114; spectrophotometry; Cloud-point extraction.