The Alteration of SNAIL2 and MMP-9 Protein Expression of JEG-3 Choriocarcinoma Cell by Methanolic Extract of Tamarindus Indica Fruit
Abstract
Objective: Tamarindus indica is one of the famous natural plant that has various effects on health. Recent research has begun to examine the effect of Tamarindus indica on cell malignancy. One of the things that happens in cell malignancy is an increasing ability of cell migration, which is characterized by increasing expression of Snail2 and MMP-9. This study was conducted to determine the effect of extract of Tamarindus indica Fruit Extract (TIFE) towards Snail2 and MMP-9 expression in JEG-3 cells.
Methods :.TIFE were extracted by using methanol. JEG-3 cells were grown in culture medium treated with TIFE (at 0, 25, 50 and 100 µg/ml). Immunofluorescence staining was carried out, and observed under Confocal Laser Scanning Microscope.
Result: Snail2 expression after treatment with TIFE 50 and 100 µg/ml higher than control- (P=0.015 ; P=0.001) whereas at MMP-9 expression there was a biphasic pattern, in which the MMP-9 expression peaked at concentration of 50 µg/ml (P=0.002), then MMP-9 expression decreased at 100 µg/ml concentration. Conclusion : The expression of Snail2 influence MMP-2 expression after treat with TIFE, so it might increase the potential for JEG-3 cell migration
Keywords : Methanolic extract, Tamarindus indica, Snail2, MMP-9
Full Text:
PDFReferences
Soemardji, A.A. (2007) Tamarindus indica L. or “Asam Jawa”: The Sour but Sweet and Useful. Available from www.inm.u-toyama.ac.jp/jp/nennpo/07nennpo/07review_article.pdf
Adeola, A.A.; Adeola, O.O.; Dosumu, O.O. (2010) Comparative Analyses of Phytochemicals and Antimicrobial Properties of Extracts of Wild Tamarindus indica Pulps. Afr. J. Microbiol. Res. Vol. 4(24) pp. 2769-2779
Bhadoriya, S.S.; Ganeshpurkar, A.; Narwaria, J.; Rai, G; Jain, A.P. (2011) Tamarindus indica : Extent of Explored Potential. Phcog Rev 2011;5:73-81
Aravind, A.R.; Joseph, M.M.; Varghese, S.; Balaram, P.; Sreelekha, T.T. (2011) Antitumor and Immunopotentiating Activity of Polysaccharide PST001 Isolated from the Seed Kernel of Tamarindus indica: An InVivo Study in Mice. The Scientific World Journal Vol 2012
Lee, K and Nelson, C.M. (2012) New Insights into the Regulation of Epithelial-Mesenchymal Transtition and Tissue Fibrosis. International Review of Cell and Molecular Biology, Vol 294: 171-221
Nie, W. and Deters, A.M. (2013). Tamarind Seed Xyloglucans Promote Proliferation and Migration of Human Skin Cells through Internalization via Stimulation of Proproliferative Signal Transduction Pathways. Dermatology Research and Practice Volume 2013, Article ID 359756
Chong, U.R.W.; Abdul-Rahman, P.S.; Abdul-Aziz, A.; Hashim, O.H.; Junit, S.M. (2012) Tamarindus indica Extract Alters Release of Alpha Enolase, Apolipoprotein A-I, Transthyretin and Rab GDP Dissociation Inhibitor Beta from HepG2 Cells. PLoS ONE 7(6): e39476. doi:10.1371/journal.pone.0039476
Ueda, S.M.; Mao, T-L.; Kuhajda, F.P.; Vasoontara, C.; Giuntoli, R.L.; Bristow, R.E.; Kurman, R.J. et al. (2009) Trophoblastic Neoplasms Express Fatty Acid Synthase, Which May Be a Therapeutic Target via Its Inhibitor C93. The American Journal of Pathology, Vol. 175, No. 6
Pulkka, O.P., Nilsson, B., Sarlomo-Rikala, M., Reichardt, P., Eriksson, M., Hall, K.S., Wardelmann, E. et al. (2017) SLUG transcription factor: a pro-survival and prognostic factor in gastrointestinal stromal tumour. British Journal of Cancer 116, 1195–1202
Komutarin, T.; Azadi, S.; Butterworth, L.; Keil, D. Chitsomboon, B.; Suttajit, M., and Meade B.J. (2004) Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo. Food and Chemical Toxicology Vol. 42 pp 649-658
Bhutkar, M.A. and Bhise, S.B. (2011) Anti-Oxidative Effect of Tamarindus indica in Alloxan Induced Diabetic Rats. International Journal of Research in Pharmaceutical and Biomedical Sciences. Vol. 2 (3). pp 1006-1009
Luzia, D.B.M., Jorge, N. (2011) Antioxidant activity, fatty acid profile and tocopherols of Tamarindus indica L. seeds. Ciênc. Tecnol. Aliment., Campinas, 31(2): 497-501
Aravind, S.R.; Joseph, M.M.; Varghese, S.; Balaram, P.; Sreelekha, T.T. (2012) Polysaccharide Pst001 Isolated From The Seed Kernel Of Tamarindus indica Induces Apoptosis In Murine Cancer Cells. International Journal Of Life Science & Pharma Research Vol 2 Issue 1
Steinestel, K.; Eder, S.; Schrader, A.J. and Steinestel, J. (2014)Clinical significance of epithelial-mesenchymal transition. Clinical and Translational Medicine 3:17
Conacci-Sorrell, M, Simcha, I., Ben-Yedidia, T., Blechman, J., Savagner, P., Ben-Ze’ev, A. 2003. Autoregulation of E-cadherin expression by cadherin–cadherin interactions: the roles of β-catenin signaling, Slug, and MAPK. The Journal of Cell Biology, Volume 163, Number 4, pp 847–857
Eagleton, M.J., Peterson, D.A., Sullivan,V.V., Roelofs, K.J., Ford, J.A., Stanley, J.C., and Upchurch, G.R. 2002. Nitric Oxide Inhibition Increases Aortic Wall Matrix Metalloproteinase-9 Expression. JOURNAL OF SURGICAL RESEARCH VOL. 104, pp. 15-21
Sanyal, M., Nag, T.C., and Das, C. (2000) Localization of Nitric Oxide Synthase in Human Trophoblast Cells: Role of Nitric Oxide in Trophoblast Proliferation and Differentiation. American Journal of Reproductive Immunology 43:70–77
Akool, E., Doller, A., Mu¨ ller, R., Gutwein, P., Xin, C., Huwiler, A., Pfeilschifter, J., and Eberhardt, W. (2005) Nitric Oxide Induces TIMP-1 Expression by Activating the Transforming Growth Factor β-Smad Signaling Pathway. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 280, NO. 47, pp. 39403–39416
Refbacks
- There are currently no refbacks.
