Probiotic potential of Lactobacillus plantarum with the cell adhesion properties

Abhinandan Patil

Abstract

The use of microbes as functional food maintain health and prevent many disease and disorder. The isolation and research of new strains of probiotic strains especially lactobacilli proven to be useful to satisfy the increasing demand of the population. In the current study, the probiotic potential of Lactobacillus strains isolated from sheep milk was investigated. The Lactobacillus strains were identified and evaluated for tolerance against gastric acidity and bile toxicity, along with the adhesion to HT-29 cells. The study proved the antimicrobial activities and antibiotic susceptibility. Survival of the strains through the host intestine was examined by the 12 week Wister rat feeding and faecal analysis trial. The in-vivo trials not only proved the adhesion but also the survival of the Lactobacillus plantarum MCC 3595 inside the intestinal lumen of the host. Thus, the isolated strain can act as the functional food by the further clinical investigation. 

Full Text:

PDF

References

I Adlerberth, S Ahrne, ML Johansson, G Molin, LA Hanson, Wold AE (1996) A mannose specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Appl Environ Microbiol 62: 2244–2251

C Ahn, DC Thompson, C Duncan, ME Stiles (1992) Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus plantarum ca TC2R. Plasmid 27: 263–264

M Alander, R Satokari, R Korpela, M Saxelin, T Vilpponen-Salmela, T Mattila-Sandholm, A von Wright (1999) Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl Environ Microbiol 65: 351–354

SF Altschul, MS Boguski, W Gish, JC Wootton (1994) Issues in searching molecular sequence databases. Nat Genet 6(2) :119–129

DG Anderson, LL McKay (1983) Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol 46 :549–552

H Annuk, J Shchepetova, T Kullisaar, E Songisepp, M Zilmer, M Mikelsaar (2003) Characterization of intestinal lactobacilli as putative probiotic candidates. J Appl Microbiol 94: 403–412

Y Azuma, M Sato (2001) Lactobacillus casei increases the adhesion of Lactobacillus gasseri NY0509 to human intestinal Caco-2 cells. Biosci Biotechnol Biochem 65: 2326–2329

AW Bauer, WM Kirby, JC Sherris, M Turk (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496

NCCLS Standard (1997) Methods for antimicrobial susceptibility testing for anaerobic bacteria. Approved Standard-Fourth edition document, vol. 11, no. 17

Chan RC, Reid G, Irvin RT, Bruce AW, Costerton JR (1985) Competitive exclusion of uropathogens from human uroepithelial cells by Lactobacillus whole cells and cell wall fragments. Infect Immun 47: 84–89

Coconnier MH, Bernet MF, Kerne´is S, Chauvie`re G, Fourniat J, Servin AL (1993) Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion. FEMS Microbiol Lett 110: 299–305

MC Collado, J Meriluoto, SSalminen (2007) Development of new probiotics by strain combinations: is it possible to improve the adhesion to intestinal mucus? J Dairy Sci 90: 2710–2716

JH Cummings, JM Antoine, F Azpiroz, R Bourdet-Sicard, P Brandtzaeg, PC Calder, GR Gibson, F Guarner, E Isolauri, D Pannemans, C Shortt, S Tuijtelaars, B Watzl (2004) Pass-claim—gut health and immunity. Eur J Nutr [Suppl 2] 43: II/118-II/173

W Fang, M Shi, L Huang, Y Wang (1996) Antagonism of lactic acid bacteria towards Staphylococcus aureus and Escherichia coli on agar plates and in milk. Vet Res 27:3–12

FAO/WHO (2001) Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a joint FAO/WHO expert consultation, Cordoba

MF Ferna´ndez, C Boris S, Barbe´s (2003) Probiotic properties of human Lactobacillus strains to be used in the gastrointestinal tract. J Appl Microbiol 94: 449–455

Fons M, Hege T, Ladire M, Raibaud P, Ducluzeau R, Maguin E (1997) Isolation and characterization of a plasmid from Lacto-bacillus fermentum conferring erythromycin resistance. Plasmid 37:199–203

A Patil, J Disouza, S Pawar (2019) Shelf life stability of encapsulated lactic acid bacteria isolated from Sheep milk thrived in different milk as natural media. Small Rumin Res 170: 19-25.

A Patil, J Disouza, S Shinde (2015) Lactobacillus Model Moiety a New Era Dosage Form as Nutraceuticals and Therapeutic Mediator, in: Biotechnol. Bioforensics, Springer, Singapore, 11–21

K Kimura, AL McCartney, MA McConnell, GW Tannock (1997) Analysis of fecal populations of bifidobacteria and lactobacilli and investigation of the immunological responses of their human hosts to the predominant strains. Appl Environ Microbiol 63(9): 3394–3398

S Mueller, K Saunier, C Hanisch, E Norin, L Alm, T Midtvedt, A Cresci (2006) Differences in fecal microbiota in different European study populations in relation to age, gender, and country: a cross-sectional study. Appl Environ Microbiol 72(2): 1027–1033

G Reid, AW Bruce, JA McGroarty, KJ Cheng, JW Costerton (1990) Is there a role of lactobacilli in prevention of urogenital and intestinal infection? Clin Microbiol Rev 3: 335–344

CN Jacobsen, V Rosenfeldt, AE Hayford, PL Møller, KF Michaelsen, A Pærregaard, B Sandstrom, M Tvede, M Jakobsen (1999) Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl Environ Microbiol 65: 4949–4956

G Klein, C Hallmann, IA Casas, J Abad, J Louwers, G Reuter (2000) Exclusion of vanA, vanB and vanC type glycopeptide resistance in strains of Lactobacillus reuteri and Lactobacillus rhamnosus used as probiotics by polymerase chain reaction and hybridization methods. J Appl Microbiol 89: 815–824

S Silvi, MC Verdenelli, C Orpianesi, A Cresci (2003) EU project Crownalife: functional foods, gut microflora and healthy ageing. Isolation and identification of Lactobacillus and Bifidobacterium strains from faecal samples of elderly subjects for a possible probiotic use in functional foods. J Food Engin 56: 195–200

Refbacks

  • There are currently no refbacks.